MMG 301 Study Guide - Final Guide: Endoplasmic Reticulum, Cell Membrane, Eukaryote

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harlequineagle50

17 Jul 2019

School

MSU

Department

Microbio & Molecular Genetics

Course

MMG 301

Professor

N.Hammer

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Document Summary

Agar a polysaccharide found in seaweed used to prepare solid culture media. Plate count determines the number of viable cells in a culture. Bacterial isolation technique of spreading a droplet of culture or sample over the surface of a solid medium using an inoculating loop. Macroscopic cluster of cells appearing on a solid medium arising from a single cell fastidious requiring special nutritional or environmental conditions for growth-applies to bacteria. The 70s ribosomes of eukarya form part of the evidence for the endosymbiont theory. Oxidation of proteins is achieved most readily in a hot air oven. with metal solutions. by moist heat. by moist heat under pressure. in the presence of organics. in hot air oven. Which of the following methods of enumerating cells is the only one to exclusively detect live cells? coulter counter turbidty flow cytometer viable plate count direct microscopic count viable plate count. Protists are more susceptible to killing by penicillin than gram-negative bacteria.

Related Questions

In a quadrant streak, why do you intersect the previous lines and not the ends of the streak?

Select one:

a. More cell amount variability at ends, usually higher cell population

b. Keep streaks in the middle of the plate

c. Prevent cell death

d. To maintain a mixed culture

e. To count CFUs

How should you hold the inoculating loop?

Select one:

a. Maintain it at an angle at all times

b. In a test tube rack at all times

c. Like a pencil

d. Balanced on top of the tube

e. With dish gloves

Which of the following is used in the spread plate and not the streak plate method?

Select one:

a. Glass spreading rod

b. Flame loop

c. Agar plate

d. Bunsen burner/sterile utensil

e. Starting culture to sample

What would likely happen if the inoculum was not spread adequately over the agar surface?

Select one:

a. Contamination

b. Degradation

c. Sterilization

d. Uneven distribution

e. Cells will not grow

You have a mixed culture of unknown bacteria and want to identify one of the species by several subsequent experiments (needing multiple colonies of a pure culture). You do a quadrant streak and transfer to a sterile liquid medium. What next step makes the most sense?

Select one:

a. Perform a Gram stain

b. Note all colony morphology characteristics

c. Use the spread plate method or new streak plate on an isolated colony of the plate

d. Quantify the cells

e. Repeat using the other two methods of isolation

What would likely occur if the flame loop was not sterilized between streaks on the quadrant streak method?

Select one:

a. Bacteria will not stick to the loop

b. Virus will be introduced

c. Flame loop will be too hot

d. The sample will be contaminated

e. Subsequent streaks would have more colonies

Why is the glass rod in the spread plate method put in alcohol and ignited instead of sterilized like a flame loop?

Select one:

a. Glass oxidizes

b. Glass melts

c. Glass takes longer to cool

d. Glass burns

e. Glass is more sterile

MMG 301 Study Guide - Final Guide: Endoplasmic Reticulum, Cell Membrane, Eukaryote

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