MMG 301 Study Guide - Final Guide: Clostridium Botulinum, Salmonella Enterica Subsp. Enterica, Bile Acid
17 Jul 2019
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Encourages certain microbes to grow (selects these while actively discouraging others) Used to identify clostridium botulinum in food, this medium contains the antibiotics sulfadiazine + polymyxin sulphate which are added to the agar with cultures of various clostridium species, it inhibits all of them except c. botulinum. Contains ingredients which undergo an observable change (ie. dye indicated ph change by changing colour) when a particular biochemical reaction takes place. this allows you to differentiate various species amongst colonies on the same place in a mixed culture. Is selective in that the presence of crystal violet and bile salts in the medium inhibit. Gr+ organisms, only gr - organisms grown on it. Is differential due to the presence of dye (neutral red) that shows which gram organisms colonies are fermenting the lactose in the medium. *ferementers produce red colonies were non-fermenters produce no colour* Can a medium be both selective and differential.
