GENE 540 Study Guide - Midterm Guide: Reca, Escherichia Coli, Mitochondrion
Document Summary
They use pcr single molecule chain reaction; the researchers specifically used single molecule. Dna to avoid possible artifact that can occur during pcr separates in vitro recombination to isolate clear dna recombinants. If you have a mixture of molecules and during the melting step in pcr, you are separating the 2 strands then you can re- anneal one strand and another strand with a different sequence; artificial exchanges. Starting material: they had individual give a muscle sample; this individual has majority of his mtdna is 90% paternal and 10% maternal; They ran the pcr of the dna sample and looked for recombination. The observed frequency of recombinants was 0. 7%, which is 10% of all mtdna. This guy had a mixture of mtdna between maternal and paternal mtdna. Blast homology search: they predicted that if you have homologous recombination in the mitochondria, then it must have some version of the reca in the plant.
