BIOL 302 Study Guide - Midterm Guide: Alpha Helix, Beta Sheet, Protein Folding
17 Feb 2017
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These structures give silk fibers their extraordinary tensile strength. This can produce an extended protein filament: ex. Dna ligase binds 2 dna strands together: isomerase- catalyzes rearrangement of bonds in single molecule, polymerase- catalyzes polymerization reaction. Each phosphate group carries 2 negative charges, so attracts cluster of positively charged amino acid side chains, thus causing conformational change. Primary antibodies specific for protein of study are used: used to measure protein expression levels and modifications and look at protein interactions, isoelectric focusing. Isoelectric point- characteristic ph at which protein as no net charge. Proteins are electrophoresed in a narrow tube of polyacrylamide gel in which ph gradient is established by mixture of special buffers. Each protein moves to point in ph gradient that corresponds to its isoelectric point: 2d polyacrylamide gel electrophoresis, complex mixtures of proteins can"t be resolved on 1d gels, but 2d gel electrophoresis combines 2 diff separation methods.
