[BIOC 405] - Final Exam Guide - Comprehensive Notes for the exam (140 pages long!)
Document Summary
*-specifically mentioned during lecture as necessary knowledge for exam. Proteins differ tremendously in size and properties. Several properties are useful for protein purification. Specific properties are used for protein characterization. Mass spectrometry is a powerful analytical method. The rate of evolution of different proteins differs. If we look at all the proteins that exist in the universe, we"ve only samples an incredibly small fraction of the number of possible proteins. You can acquire blood, liver, muscle cells and purify the protein of interest from that source. Hence, this method is often called (cid:498)ammonium sulfate fractionation(cid:499). You have a semipermeable membrane dialysis membrane a membrane with. Dialysis is normally used for buffer exchange, where you have a protein with a particular salt that you want to remove or exchange for a different salt. very small holes. The holes are such a size that only proteins below a certain size can pass through the holes.
