BIOL 107A Study Guide - Final Guide: Eif4E, Operon, Selb
Document Summary
Lecture 10: the common features of model systems. Model organisms are relatively cheap, plentiful, easy to propagate and manipulate in the lab. Bacteria, mold, yeast, worm, flies, fish, plants, mouse, frogs, etc: the concept of transfection and transformation. Transient transfection introduced dna for a short duration. extrachromosomal or integrated. Convenient (thus used routinely), not stable, less predictable, but has less long-term secondary effect. Stable transfection relies on selecting cells by drug resistance. Plasmid has integrated into chromosome, usually in tandem copies: reporter genes, including gfp and cat. Reporter genes : gene whose rna/protein levels can be measured easily/accurately. It is used to replace other coding regions whose protein products are difficult to measure. These are activated by overexpression of a tf using cotransfection assay . interactions. Gfp is used to find the intracellular fate of a gene product or dna-protein. Cat (chloramphenicol acetyltransferase) catalyzes acetylation of chloramphenicol (obviously).
