CHEM 345 Midterm: CHEM345 Washington State Test2

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15 Feb 2019
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5" to 3" direction: rna polymerases bind to ribosomes to allow transcription, a poly a tail is added to the 3" end of messenger. This may be coded by the element itself, or independently: minimal medium- the simplest medium which will support the growth of wild type bacteria, normally containing glucose + simple salts. 5 problems (40 points total: (7 pts. ) Chemicals which cause mutations almost always cause cancer. Direct mutagens but require activation to a mutagenic forms by enzymes in the liver of animals. Determine the gene order (i. e. , which gene is in the middle) from the following data set from a transduction experiment. Bacteria were selected for the presence of a+. B is closer to a than c is, based on the cotransduction frequencies. The fact that transduction with c almost always carries b along suggests that it is beyond b from a on the chromosome. There is one apparent double crossover event: (4 pts. )