BIO374H5 Chapter Notes - Chapter 6: Dicamba, Tobacco Mosaic Virus, Plasmid
Document Summary
Chapter 6: plant biotechnology: uses of biotech to enhance selective breeding. Marker-assisted selection (mas) sample tissue to test for presence of markers inherited with alleles for desired traits (productivity/disease resistance); also used to select for traits that are difficult to measure chemicals/radiation pool of available traits. Mutation breeding exposing seeds to mutation-causing. Protoplast fusion regenerate hybrid plant (polyploid=larger product) Plant transformation insert new genes into target plant . Agrobacterium (soil bacterium) used to insert genes into plants transgenic: genetic engineering of plants. T-dna of ti (tumour-inducing) plasmid modified to remove + replace genes that cause tumour formation w/ desired genes. Leaf fragment technique small discs cut from leaf cultured briefly in a medium containing genetically modified. Agrobacterium ; dna from ti plasmid integrated into dna of host cell; leaf discs then treated w/ plant hormones. Gene gun shoots dna-coated pellets of gold/tungsten directly into plant cells for integration into cell"s dna.
