BIOL 2051 Chapter : Structure And Function Study Questions
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Question 1
What is the last step of successfully preparing a bacterial smear for a simple stain?
Spread cells with inoculating loop | ||
Label the slides | ||
Heat fixation | ||
Air dry the smear |
Question 2
When looking at your smear under the microscope, all you see is darkness (despite shining light through your slide)- the best explanation is:
all your bacteria are dead | ||
your smear was too thin | ||
forgot to add the bacteria | ||
your smear was too thick |
Question 3
If a microbe can ferment lactose and its fermentation products include lactic acid and CO2, then the results for this assay should be:
the broth will appear yellow and there will be a gas bubble in the Durham tube | ||
the broth will appear red and there will be no gas bubble in the Durham tube | ||
the broth will appear yellow and there will be no gas bubble in the Durham tube | ||
the broth will appear red and there will be a gas bubble in the Durham tube |
True/Flase
Question 1
If the result for this carbohydrate test is negative, then the organism had NO growth in the tube.
Question 2
The biochemical basis of the Gram stain is the thickness of peptidoglycan layers surrounding cells. Therefore a Gram positive cell with a thin layer of peptidoglycan should stain as Gram negative.
Question 3
If the iodine is not applied during the Gram stain, then gram positive cells will likely stain pink
Question 4
A simple stain allows you to see the cell shape and arrangement.
Question 5
If there were no limit to magnification, a light microscope could enlarge microbes infinitely.
Question 6
When doing a bacteria smear from a broth medium you should first place one to two loopfuls of water on the center of the slide?