BIOL 2051 Chapter : Chapter 2 Outline

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15 Mar 2019
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Size of prokaryotes: prokaryotes are generally smaller than eukaryotes, small size allows nutrients to reach all parts of the cell, prokaryotic cells can be as small as 0. 2 m. The smallest eukaryotic cells are 2 m: cocci- typically have diameter of 2 m, some microbiologists have proposed that bacteria smaller than 0. 2 m exist in nature, cells referred to as nano bacteria. Microscopy: simple microscopes, one lens, magnify 50 300x, compound microscopes, multiple lenses, much higher magnification, ones in 2051 lab magnify 1000x. Magnification: a microscope with a 100x objective and 10x ocular has a total magnification of. 1000x: this means an object will appear 1000x larger than it is, can see bacteria at this magnification, but not internal structures, cannot see viruses. Correct each other"s aberrations (imperfections: use shorter wavelengths of light. Blue light (400nm) is shortest wavelength so gives highest resolution: increase numerical aperture (measure of light gathering ability of an objective lens)

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