ECON 2010 Chapter : Chap011
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Atp has a pka value of 3. 5 while gtp has a pka value of 2. 3. Amp travels further because it only has one phosphate group. Dna treated with naoh spreads due to the breaking of the 2 strands. If run 30 times it is amplified 109 times: functions of nucleotidse, carriers of chemical energy in the cell (eg. , atp, gtp, components of cofactors (eg. , nad, fad, intermediates in cellular communication and signal transduction (eg. , camp, cgmp) Day 2: purification of pcr and restriction endonuclease digestion: we must remove dntps and taq dna polymerase in the pcr reaction or else it would refill the sticky ends generated by restriction endonucleases. It neutralizes charge on po3- group of dna, making the molecule less hydrophilic and precipitate out of the solution: 100% ethanol causes precipitation of dna. Day 3: digestion check using agarose gel electrophoresis and ligation. Be sure to know why: a strong dna band should be seen at 780 bp.
