BIOL 261 Chapter : Chapter 26 Class Notes Microbial Growth Control
15 Mar 2019
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This means the autoclave did not kill the bacteria. [remember: autoclaves are used to sterilize instruments for surgery. ] Decimal reduction time (d): amount of time required to reduce viability tenfold: temperature = time to kill. Autoclave: a sealed device that uses steam under pressure: time and temperature are what kills organisms not pressure, bacillus stearothermophilus spore strips are used in order to quality control. Filter sterilization: filtration is the passing of a liquid through a membrane, depth filters, pore size = hole , hepa: high efficiency particulate air filter, bacteria: 0. 45 micron filter, mycoplasma: 0. 22 micron filter, staph. Inoculate plate with a liquid culture of a test organism: discs containing antimicrobial agents are placed on surface of nutrient agar plate. Incubate for 24-48 hours: test organism shows susceptibility to some agents, indicated by inhibition of bacterial growth around discs (zone of inhibition)
