PSY4HNA Lecture Notes - Southern Blot, Deeper Understanding, Northern Blot
Week 9 – Theory and Research: Biological Perspective (Agnes Hazi)
Have a general understanding of the different laboratory techniques discussed in seminar
HISTOLOGY
Microscopic anatomy of cells and dead tissues
Can localise changes in the brain
Cresyl Violet - Identify neural structure in the brain
and spinal cord tissue
- Can detect damage and lesions
- Detects cell density
- Stains cell bodies but cannot see
networks or connections
Golgi Stain - Stains the entire neuron including
dendrites and axon
- Connections between neurons can
be visualised
- Complex network structure can be
investigated
- Doesn’t stain all neurons therefore
not good if looking at density
Luxol Fast Blue - Stains myelin
- Demyelination can be investigated
in the CNS
-Used in MS research
- If there is myelination, less staining
will show as there is less density
Amyloid Stain - Can detect amyloid deposits in
tissues
- Useful in Alzheimer’s Disease
- Detect amyloid plaques
- Animal and post-mortem tissue
find more resources at oneclass.com
find more resources at oneclass.com
Document Summary
Week 9 theory and research: biological perspective (agnes hazi) Have a general understanding of the different laboratory techniques discussed in seminar. Microscopic anatomy of cells and dead tissues. Identify neural structure in the brain and spinal cord tissue. Stains cell bodies but cannot see networks or connections. Stains the entire neuron including dendrites and axon. Doesn"t stain all neurons therefore not good if looking at density. Demyelination can be investigated in the cns. If there is myelination, less staining will show as there is less density. Antibodies are used to bind to antigens. The body will develop antibodies in response to foreign proteins that form. Compiling data sets from multiple cell investigations allows scientists to better understand, predict and influence the factors that underlie cell health, proliferation, function and death. Optical technique used to resolve structures beyond the resolution of conventional light microscopy. Fine mapping of cellular structures such as neural systems, golgi apparatus and nuclear membranes.
