BIOM20001 Lecture 25: 25 Environmental and genetic interactions B
Document Summary
We disconnect the eve enhancer region from what it normally regulates and make it enhance our goi or marker gene which allows us to easily determine how it enhances (i. e. where in the embryo it induces gene expression) Entire region can be cloned and placed next to gal4, which would visually mark the wt expression pattern with gfp. Or, enhancer regions can be dissected into various control elements by taking different regions and examining their expression patterns. Organisms need to be able to detect compounds in their environment be they tasty or toxic. One response to external compounds can be induction of gene expression. Understanding how these compounds effect expression levels can tell us about a particular gene"s function. Metallothioneins are proteins able to bind to compounds, ie metals. To study the interaction metallothioneins with copper it is possible to use the two part. Metallothionein b enhancer region is connected to gal4.
