BIO1022 Lecture Notes - Lecture 3: Reverse Transcriptase, Genomic Library, Dna Replication
Lecture 3 (3/8): Molecular Biology
DNA: DNA replication
· Chromosomes = DNA and protein
Prokaryote & Eukaryotes
· Prokaryote and Eukaryotes differ in their replication
· This is due simplicity of their bacteria
· Origin of replication: where the copy of DNA starts
Replication fork
· Moves in both directions
· DNA grows in a 5’ à 3’ direction
· DNA polymerase 3 replicates DNA
Leading strand & Lagging strand
· Leading strand: Can add bases on the 3’ end going towards the replication fork
DNA ligase
· Sticks things together
Modification of an organism’s DNA
Lecture 4 (5/8): Molecular Analysis
Gene Technology/Genetic Engineering
Molecular cloning
· Genomic DNA: gDNA
·
Technique 1: Restriction Enzymes