BIO1022 Lecture Notes - Lecture 13: Pasteur Pipette, Amylase, Mortar And Pestle
13 Oct 2018
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You have performed the experiment (your practical 3, metabolism experiment). You wish to tell your friend about this experiment so they can replicate it exactly. Took ten germinating seeds and weighed their weight. Then, used mortar and pestle to crush seeds into the mud. Slowly added 10 ml buffer to further smash the seeds which let amylase enter it. Filtered the seeds being crushed into a beaker and poured the amylase was poured into the measuring tube. Diluted the extract by a straw containing 5 ml of amylase extract solution and 20 ml buffer. This mixture was then compared to the control group placed in the hot water at 80 c for 10 minutes, which denatured the enzymes i(cid:374)side the test tu(cid:271)e a(cid:374)d (cid:272)ould(cid:374)"t react with the starch and (cid:449)ould(cid:374)"t reach the achromic point. For another reason, the e(cid:374)zy(cid:373)es did(cid:374)"t totally de(cid:374)ature (cid:449)hi(cid:272)h (cid:272)a(cid:374) (cid:271)e sol(cid:448)ed (cid:271)y putti(cid:374)g i(cid:374) the (cid:449)ater (cid:271)ath for a longer time.
