BMS1062 Lecture Notes - Lecture 7: Proinsulin, Disulfide, Thermodynamic Cycle
14 Aug 2018
School
Department
Course
Professor
Document Summary
Objectives: identify the 5 stages of protein synthesis describe the mechanism of each stage recognise the role rrna in each stage understand how and why the process differs in eukaryotes and prokaryotes. Initiation: activation of trna ( loading of amino acids or charging trna, elongation, termination, post-translational modifications. Eukaryotic and bacterial ribosomes have similar structures and functions, being composed of one large and one small subunit. When not actively synthesizing proteins, the 2 subunits of the ribosome are separate (60s and 40s in eukaryotes, 50s and 30s in prokaryotes) S stands for svedberg units, ie the rate at which these subunits sediment, so naturally 60s being the larger subunit would sediment at a faster rate) In protein synthesis, ribosomes provide the framework for protein synthesis in the cytoplasm ribosomes are made up of ribosomal rna and polypeptides remember there is 20 types of aminoacyl synthetase, one for each type of amino acid.
