BMS1011 Lecture Notes - Lecture 1: Chymotrypsin, Hydrolysis, Cyanogen Bromide
24 Aug 2018
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Document Summary
Amino acids: a compound that contains both an amino group (-nh2) and a carboxyl (-cooh) group. Amino acids: an amino acid which the amino group is on the carbon adjacent to the carboxyl group. Can be assigned d- or l- configuration when drawn in fischer projection form. Isoelectric point (pi): the ph which zwitterion is. Titration of neutral amino acid glycine with sodium hydroxide pka1 = acid ph = pi (no net charge) pka2 = basic (where sodium hydroxide was added more) Ninhydrin common reagent used to detect amino acids. Peptides: short polymer of amino acids joined by peptide bones; classified by number of amino acids in the chain. Dipeptide: two amino acids joined by peptide bond. Tripeptide: three amino acids joined by peptide bond, etc. Polypeptide: a macromolecule containing many amino acids joined by peptide bonds. Brcn used to cleave peptide bonds formed by carboxyl group of methionine. Peptidases hydrolyse specific peptide bonds at c-terminal (carboxyl end)
