BIOL3006 Lecture Notes - Lecture 1: Proteomics, Centromere, Sister Chromatids
Document Summary
Most cells only have one nucleus: red blood cells (rbc) don"t have a nucleus. Some cells have multiple nuclei: protozoa, fungi, syncytium (skeletal muscle, myoblast (precursor cell) fusion, cancer cells, may have benefits for the cancer cell, binucleate (2 nuclei, micronuclei (small, extra nuclei which are separate from primary nucleus) Nuclear pore complex (npc: regulates import and export of molecules. Each chromosome has its own location in the nucleus: usually lines up on the outside, when genes are expressed, they migrate to the middle. Mrna (messenger: following transcription of gene (often undergoes splicing, undergoes translation at ribosomes. Non-coding rnas the introns: don"t encode proteins but are involved in other functions (eg. telomere synthesis) Can use dapi dye to bind to nucleic acids to show chromatin. Immunofluorescence (if) is an important lab technique: specific ab"s are used to target protein of interest, ab can be directly used as a flurophore, or a secondary ab (with a tag) can be used.
