ANAT 262 Lecture Notes - Lecture 1: Nucleolus Organizer Region, Nuclear Membrane, Lamin B2

• School: McGill University
• Department: Anatomy & Cell Biology
• Course: ANAT 262
• Professor: Ursula Stochaj

Document Summary

Npc at junctions of inner & outer nuclear membranes. Nuclear bodies: smaller compartments, detected by immunofluorescent staining (antibody pattern) f"n of nuclear body (e. g. storage of splicing factors) Defined by immunolocalization studies, have diverse functions (e. g. play role in splicing) Nuclear matrix: made of filaments & other components, what is left over when nucleus extracted w/detergent, salt, rnas, dnas. Filamentous components help organize nuclear f"ns (e. g. splicing, replication) Er can communicate w/nuclear membrane (nuclear envelope) trafficking of material across. Can control function of npc by using ions released in er e. g. cell receives outside signal er releases ion ion in er lumen travel to perinuclear space. Control npc (at junction of outer & inner nuclear membrane) Nucleolus involved in ribosomal biogenesis: makes large & small subunits. Different rrnas in large & small subunits, some synthesized in nucleolus (5s rna transcribed in nucleus but outside of nucleolus) Proteins from cytoplasm nucleus nucleolus to make ribosomal subunits.