BIOL1040 Lecture Notes - Lecture 21: Inner Mitochondrial Membrane, Glucokinase, Citric Acid Cycle

24 views3 pages

rubyzebra185

23 May 2018

School

University of Newcastle

Department

Biological Sciences

Course

BIOL1040

Professor

Bill

For unlimited access to Class Notes, a Class+ subscription is required.

GLYCOLYSIS

LEARNING GOALS

• Glycolysis

o Understanding of overall pathway

o What are the products of glycolysis – Control points

o Key reactions

• Where to after glycolysis (later lectures)

o Lactic acid fermentation

• Understanding of overall pathway

• What conditions does it occur

o TCA cycle

o Alcoholic fermentation

• Overall reaction of the breakdown of glucose

o C6H12O6 + 6O2 ® 6CO2 + 6H2O

o ΔG for this reatio is -2820 kJ mol-1

o Synthesis of more than 30 ATP molecules from ADP and Pi

• Glycolysis

o Glucose (6 carbon) ® pyruvate (3 carbon)

o 2 ATP and reduced 2NAD+ to 2NADH

o cytosol

• Citric acid cycle (TCA, Krebs cycle)

o Pyruvate converted to CO2

o ATP, NADH, FADH

o Mitochondria

• Electron transport chain (oxidative phosphorylation)

o Utilises NADH and FADH to produce ATP

o Generates the bulk of ATP

o Inner mitochondrial membrane

o Is where O2 is consumed

• Glycolysis is the central pathway of metabolism

o Found in almost all organisms

GLYCOLYSIS

• Generates

o 2 ATP

o 2 NADH

• NADH is oxidised in the mitochondria, generates NAD+ which returns to the cytosol for

glycolysis

• Pyruvate goes into the mitochondria

• Where does this glucose come from?

o Dietary uptake

o Glycogen (glycogenesis)

• Glycolysis can be broken down into energy investment stage and an energy recover stage

REACTION 1 - HEXOKINASE OR GLUCOKINASE

• Glucose + ATP ® Glucose-6-phosphate

• Catalysed by hexokinase or glucokinase

• Why invest energy in an energy generation process?

o Phosphorylation keeps glucose in the cell

find more resources at oneclass.com

Unlock document

This preview shows page 1 of the document.
Unlock all 3 pages and 3 million more documents.

Already have an account? Log in

Related Questions

Fill in the blank in the following table:

metabolic cycle	location pro/ eukaryote	components	substrates	products	pathway purpose
embden- meyerhof- parnas (glycolysis)	cytoplasm/ cytoplasm	10 enzymes
pentose phosphate pathway	cytoplasm/ cytoplasm	dozen enzyme	1 Glucose (6 carbon), 1 NADP, 1ATP	1 Ribose-P (5 carbon) + 1CO2, 1NADPH, or sugars, 1 ADP	Nucleotide synthesis, NADPH for anabolism
PDH reaction	cytoplasm/ mitochondria matrix
TCA/ Krebs/ citric acid cycle					generation of high energy electron carriers, provide metabolic precursors
Electron transport chain + ATP synthase (aerobic respiration)
Electron transport chain + ATP synthase (anaerobic respiration)			1Nitrate or sulfate or CO2, 1NADH + ____ ADP Or 1FADH2+ ____ ADP	N2, or NO2 or H2S or CH4 1NAD + ____ ATP Or 1FADH2+ ____ ATP	Generate proton motive forces abd use to generate ATP regenerate NAD+
Fermentation	cytoplasm/ cytoplasm	1 or more enzymes (vary by organism)	1 pyruvate, 1 NADH	1 alcohol or acid (maybe 1 CO2) NAD+	Regenerate NAD+

QUESTION 1

Which of the following is the correct order of the three stages of cellular respiration?

	a.	Krebs cycle, glycolysis, electron transport
	b.	electron transport, glycolysis, Krebs cycle
	c.	Krebs cycle, electron transport, glycolysis
	d.	glycolysis, Krebs cycle, electron transport
	e.	electron transport, Krebs cycle, glycolysis

1 points

QUESTION 2

Consider a bacterial cell that performs anaerobic respiration. If that bacterial cell had access to six molecules of glucose to use, how many ATP molecules would it be able to produce?

	a.	2
	b.	34
	c.	36
	d.	6
	e.	12

1 points

QUESTION 3

The oxygen that organisms need to live is used exclusively in which of the following metabolic pathways?

	a.	Electron transport chain
	b.	Anaerobic respiration
	c.	Glycolysis
	d.	Krebs cycle
	e.	ATP-Synthase

1 points

QUESTION 4

The electron transport chain uses the energy in NADH and FADH₂ to pump H⁺ ions from the matrix to the innermembrane space. The H⁺ gradient is then used to power ATP-Synthase. Enough H⁺ is pumped from the energy in a molecule of NADH to produce ______ ATP molecules, while enough H⁺ is pumped from the energy in a FADH₂ molecule to produce ______ ATP molecules.

	a.	three; two
	b.	three; three
	c.	two; two
	d.	two; three

1 points

QUESTION 5

Which of the following list contains only products of cellular respiration?

	a.	glucose, oxygen, ADP
	b.	ATP, carbon dioxide
	c.	oxygen, ADP, carbon dioxide
	d.	ATP, glucose, oxygen
	e.	glucose, ATP

1 points

QUESTION 6

What powers the enzyme ATP-Synthase?

	a.	NADH molecules
	b.	oxygen (O₂)
	c.	ATP
	d.	NADH and FADH₂ molecules
	e.	The H⁺ gradient between the matrix and inner-membrane space

1 points

QUESTION 7

Organisms exist which are able to survive using only glycolysis (i.e. without using oxidative phosphorylation).

True

False

1 points

QUESTION 8

Each ADP molecule contains ________ phosphates, and each ATP molecule contains ________ phosphates.

	a.	three; three
	b.	two; two
	c.	one; two
	d.	two; three
	e.	one; three

1 points

QUESTION 9

Adding a phosphate group to ADP to form ATP is an endergonic reaction.

True

False

1 points

QUESTION 10

The majority of ATP produced during cellular respiration is produced during glycolysis.

True

False

1 points

QUESTION 11

Glycolysis occurs:

	a.	on enzymes located in the inner-membrane.
	b.	in the cytosol.
	c.	in the inner-membrane space.
	d.	in the nucleus of the cell.
	e.	in the matrix of mitochondria.

1 points

QUESTION 12

Muscle soreness associated with strenuous exercise is due to:

	a.	the production of alcohol during anaerobic respiration.
	b.	a buildup of lactic acid.
	c.	an excessive amount of ATP that builds up during exercise.
	d.	the large amounts of CO₂ building up in the muscle.

1 points

QUESTION 13

A required reactant for glycolysis is:

	a.	the electron transport chain.
	b.	carbon dioxide.
	c.	oxygen.
	d.	NAD+.
	e.	mitochondria.

1 points

QUESTION 14

NADH is produced in all of the following except:

	a.	the electron transport chain.
	b.	the Krebs cycle.
	c.	glycolysis.

Question 1 Organisms exist which are able to survive using only glycolysis (i.e. without using oxidative phosphorylation).
True
False

Question 2 Glycolysis occurs:
a. in the matrix of mitochondria.
b. in the inner-membrane space.
c. in the cytosol.
d. in the nucleus of the cell.
e. on enzymes located in the inner-membrane.

Question 3 Muscle soreness associated with strenuous exercise is due to:
a. the large amounts of CO2 building up in the muscle.
b. an excessive amount of ATP that builds up during exercise.
c. a buildup of lactic acid.
d. the production of alcohol during anaerobic respiration.

Question 4 Each ADP molecule contains ________ phosphates, and each ATP molecule contains ________ phosphates.
a. two; three
b. two; two
c. three; three
d. one; three
e. one; two

Question 5 The oxygen that organisms need to live is used exclusively in which of the following metabolic pathways?
a. Glycolysis
b. Electron transport chain
c. Krebs cycle
d. Anaerobic respiration
e. ATP-Synthase

Question 6 NADH is produced in all of the following except:
a. the electron transport chain.
b. glycolysis.
c. the Krebs cycle.

Question 7 Consider a bacterial cell that performs anaerobic respiration. If that bacterial cell had access to six molecules of glucose to use, how many ATP molecules would it be able to produce?
a. 36
b. 6
c. 12
d. 34
e. 2

Question 8 The majority of ATP produced during cellular respiration is produced during glycolysis.
True
False

Question 9 What powers the enzyme ATP-Synthase?
a. The H+ gradient between the matrix and inner-membrane space
b. ATP
c. oxygen (O2)
d. NADH molecules
e. NADH and FADH2 molecules

Question 10 Which of the following list contains only products of cellular respiration?
a. ATP, glucose, oxygen
b. ATP, carbon dioxide
c. oxygen, ADP, carbon dioxide
d. glucose, ATP
e. glucose, oxygen, ADP

Question 11 Adding a phosphate group to ADP to form ATP is an endergonic reaction. True False

Question 12 The electron transport chain uses the energy in NADH and FADH2 to pump H+ ions from the matrix to the innermembrane space. The H+ gradient is then used to power ATP-Synthase. Enough H+ is pumped from the energy in a molecule of NADH to produce ______ ATP molecules, while enough H+ is pumped from the energy in a FADH2 molecule to produce ______ ATP molecules.
a. two; three
b. three; three
c. three; two
d. two; two

Question 13 Which of the following is the correct order of the three stages of cellular respiration?
a. glycolysis, Krebs cycle, electron transport
b. Krebs cycle, electron transport, glycolysis
c. electron transport, Krebs cycle, glycolysis
d. electron transport, glycolysis, Krebs cycle
e. Krebs cycle, glycolysis, electron transport

Question 14 A required reactant for glycolysis is:
a. carbon dioxide.
b. mitochondria.
c. NAD+.
d. the electron transport chain.
e. oxygen.

maroonguinea-pig450

BIOL1040 Lecture Notes - Lecture 21: Inner Mitochondrial Membrane, Glucokinase, Citric Acid Cycle

Get access

Related Documents

APK 2105C Lecture Notes - Lecture 13: Acetyl-Coa, Citrate Synthase, Oxidative Phosphorylation

NSCI 1413 Lecture Notes - Lecture 8: Electronegativity, Atp Synthase, Chemiosmosis

BIS 2A Lecture Notes - Lecture 4: Oxidoreductase, Coenzyme Q10, Glycolipid

Related Questions