PHAR2210 Lecture Notes - Lecture 7: Mercaptopurine, Drug Metabolism, Untranslated Region
Document Summary
Coding regions within the eukaryotic gene carry the nucleotide sequence that gives rise to the amino acid sequence of the arising protein. Sequence of bases- made up of triplets and each triplet has a specification to an amino acid within the coding region: made up of introns (not transcribed into mrna) and exons (that finds its way into mrna) Each of these regions have a degree for variability in them: variability is not great- differ that in 1 in 1200 bases differ between 2 humans. If differences in coding region= different protein activity. Codon: three nucleotide sequence that codes for a specific amino acid. Interaction, response and toxicity: why animals and humans respond differently to the same drug, hence sources of variability: (as above) Example: coding region polymorphism in an important drug metabolising enzyme: Thiopurine methyltransferase: e. g. azathiopurine and mercaptopurine, azathioprine: prodrug, is tolerable than mercaptopurine, mercaptopurine is a pruine sulfur= mercapto, on the 6th position.
