BIOL1004 Lecture Notes - Lecture 11: Polymerization, Globular Protein, Tubulin

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Microtubules: microtubules are hollow cylinders 25nm in diameter, they are composed of a globular protein called tubulin, tu(cid:271)uli(cid:374) e(cid:454)ists i(cid:374) t(cid:449)o for(cid:373)s, -tu(cid:271)uli(cid:374) a(cid:374)d -tubulin, i(cid:374) solutio(cid:374), o(cid:374)e su(cid:271)u(cid:374)it of -tu(cid:271)uli(cid:374) asso(cid:272)iates (cid:449)ith o(cid:374)e su(cid:271)u(cid:374)it of - tubulin to form a heterodimer. Tubulin protofilaments form the microtubule: tubulin dimers bind end-to-end to form protofilaments, thirteen protofilaments associate to form the wall of the microtubule. Microtubule dynamics: microtubules are highly dynamic structures, they grow (polymerise) through addition of tubulin dimers or shorten (depolymerise) through loss of tubulin dimers. Microtubule organisation: microtubule assembly, disassembly and organisation are regulated by specific proteins called microtubule associated proteins (maps), microtubule polymerisation usually occurs at specific microtubule nucleating sites. They do(cid:374)"t just ra(cid:374)do(cid:373)l(cid:455) group i(cid:374) a (cid:272)ell! Microtubule nucleation at the centrosome: most animal cells have a single microtubule nucleating site called the centrosome located near the nucleus, one end of the microtubule is embedded in the centrosome and the microtubule grows out into the cytoplasm.

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