BIOL2174 Lecture 13: Mechanisms of ABC pumps
Document Summary
In many families - it"s a superfamily of proteins. Import, export, ion channels, dna repair & receptors (only a small group is used for dna repair processes) Single chains, or smaller chains that can be assembled after translation into a larger protein. Substrate access - capture from periplasm, via membrane & cytosolic routes. Minimal topology = (2 x nbd) + (2 x tmd) Must have an nbd to be part of this family. Gxxgxgkss/t; hhhhd walker a/b = atp binding. You must have this to be abc, but if you have it alone, it doesn"t make you abc. Coupling regions to communicate between the 2 domains of the protein. Might just have one of each of these things, or may have many more, one of each is just the minimum. Mbp binds to the top of the protein. Takes the nuclear binding domain and locks them in. Forms a sandwich dimer and traps atp in there.
