BCMB30004 Lecture Notes - Lecture 20: Intermembrane Space, Tradd, Deubiquitinating Enzyme

• School: University of Melbourne
• Department: Biochemistry and Molecular Biology
• Course: BCMB30004
• Professor: Professor Cheng

Document Summary

* necrosis occurs when you injure a cell so it is death by injury and this injury can be mechanical, chemical or hypoxia. Associated with inflammation (this is because the cell contents are released in a random way and so this can a way that the immune system realises that something bad is going on). * occurs due to withdrawal of positive survival signals (e. g. growth factors) and/or receipt of negative signals (e. g. oxidants, tnf, x-rays). * the major enzymes that mediate apoptosis are the caspases and these have cysteine in their active site and these enzymes cleave between an aspartic acid and a glutamic acid. So, cleavage is not random, they recognise a motif and they cleave after the aspartic acid. * caspases are dangerous proteins and so the cell needs to keep them in check and so they are synthesised as inactive procapsases and so they a pro domain that renders them inactive.