PHAR2823 Lecture Notes - Lecture 6: 2.5D, Thermogravimetric Analysis, Calorimetry
Document Summary
Yes can calculate concentration of particles in solution because it counts every single particle not achievable in any other method. Limitation of light microscope is that its for larger particles. The scanning electron microscope (sem) uses a focused beam of high- energy electrons focused over the sample surface. Accelerated electrons in an sem carry significant amounts of kinetic energy, and this energy is dissipated as a variety of signals produced by electron-sample interactions when the incident electrons are decelerated in the solid sample. These signals include: secondary electrons (cid:523)that produce sem images(cid:524), backscattered electrons (cid:523)bse(cid:524), diffracted backscattered electrons (ebsd that are used to determine crystal structures and orientations of minerals), photons (cid:523)characteristic x-rays that are used for elemental. Has to be dehydrated because of high vacuum. Not do hydrated molecules may shrink and look different than in reality, change conformation. Has to be conductive coat samples with gold, titanium or palladium. Sample is static frozen analysis and continuum x-rays)
