PSY3710 Lecture Notes - Lecture 1: Fluid Mosaic Model, Globular Protein, Lipid Bilayer
11 Apr 2018
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Document Summary
Around 1935: lipoid core bordered by lipid monolayers. 1960s there was the attempt to make account for new observed properties. There was static picture which was freeze fracture electron microscopy: proteins penetrate the interior. There was dynamic picture which was fluorescence microscopy labelled proteins move rapidly in the membrane plate. Freeze- fracture techniques showed bumps in the membrane interior- benson green subunit model , then fluid mosaic . Refinement of membrane models is based on new evidence. Still around today with addition and refinements. Features globular protein icebergs floating in a lipid bilayer sea . Both lipids and proteins undergo rapid lateral movement; highly dynamic structure. Experimental tools of biochemistry used at molecular level. Tlc- thin layer chromatography, sds-page, mass spectrometry. Genomics- genes, lipidomics- lipids, proteomics- protein and glycomics- carbs. The different constituent membranes of the rat hepatocyte contain different lipids. The inner and outer leaflets of the human erythrocyte membrane contain different lipid asymmetry.
