BIOL 2104 Lecture Notes - Lecture 2: Crc Press, Homomeric, Thiol

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Allozyme analysis is a technique which is used in study of genetics because it reveals the genetic variation that exists within a wide range of organisms (g mez, 1998). Allozymes are different forms of an enzyme expressed by alternative alleles on the same gene locus (micales & Analysis of these allozymes can be done by protein electrophoresis. Protein electrophoresis involves the movement of proteins within an electric field with mobility being dependent on factors such as the size and shape (secondary and tertiary structure), as well as the charge of the protein (due to primary structure). Other factors that can affect the mobility are electric-field strength, matrix ph, and ionic buffer strength of the electric field. Because there are so many factors involved in analyzing proteins during electrophoresis, it is unusual for two different proteins to have the same relative mobility (g mez, 1998).

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