BIOL 467 Lecture Notes - Lecture 4: Heterotrimeric G Protein, Scaffold Protein, Grb2
Document Summary
Comparison of mapk signaling cascades in eukaryotes. Mapk signaling in yeast is crucial for the response to mating type. In budding yeast, the cue is the pheromone the alpha factor. Its getting received by a receptor that then actually cause the dissociation of a heterotrimeric protein. One of those subunits will then go one and help to signal through this map kinase kinase kinase cascade through ste11. Paper: how they were able to determine the function of the scaffold ste5 in mediated a response to this pheromone cue. Ste5 a protei(cid:374) that (cid:449)ould (cid:374)or(cid:373)all(cid:455) e(cid:454)ist e(cid:448)e(cid:374) i(cid:374) a (cid:272)ell that"s (cid:374)ot (cid:373)ati(cid:374)g. ste5 (cid:271)e(cid:272)o(cid:373)es reall(cid:455) crucial for signaling in a cell in response to pheromone. In comparison to a cell that is not receiving that pheromone. One of those heterotrimeric g protein subunits will actually recruit ste5 to the membrane and bring it into close physical proximity with some of those map kinase kinase signaling factor.
