BIOL-205 Lecture Notes - Lecture 7: Immunofluorescence, Release Factor, Peptide Bond
15 Dec 2016
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Document Summary
At the end of this topic, you should be able to . Describe what is meant by a reading frame and why it is important. Describe the structure and role of trnas and the function of trna synthetases. Explain how release factor terminates translation them to relevant examples. Most functions in a cell are carried out by proteins. Protein sequence dictates its folding and therefore its function. Amino acids are linked by peptide bonds. Created by condensation reaction with release of water. Protein polarity is n terminal to c terminal. Nucleic acid language is translated to that of proteins 3 letters at a time. One codon = 3 nucleotides = one amino acid trna. Aminoacyl-trna synthetase links trna with amino acid. One synthetase per amino acid (not per trna!) Ribonucleoprotein complex (3 rnas, 50+ proteins: consists of a small and a large subunit. Aminoacyl trna site: next aa to be added.
