BIOL 200 Lecture Notes - Lecture 6: Tata Box, Tata-Binding Protein, Transcription Preinitiation Complex
Document Summary
Biol 200 - lecture 6, rna pol ii promoters and transcription initiation. Liquid chromatography can be used to purify a specific protein from a complex mixture of proteins. Separate proteins with antibodies that bind specifically to one protein. Identified and purified from one another using chromatography. Amanitin can bind really well to rna pol 2 and almost innactivate it. Pol 1 is not sensitive to that toxin. Pol 3 is a little bit less sensitive to that toxin. All thre of the polymerases are more ocmplex than bacterial rna polymerase but they have subunits that are homologous in structure and function to the 5 bacterial subunits. Polymerase 2 has a carboxyl terminal domain that is phosphorylated in actively transcribing polymerases. For transcription to initiate, a complex has to form called the pre-initiation complex. General transcription factors = required for transcription of every pol 2 dependent gene.
