BIOL 200 Lecture Notes - Lecture 13: Helicase, Eif4E, Eif4B
7 Jun 2016
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~100 bp downstream of 5" end) eif4b joins and stimulates helicase activity. Secondary structures have been removed and you have circular association of 5" and 3" end. Aug in the p site you have proofreading: results in hydrolysis of eif2-gtp to eif2-gdp. Go from unstable conformation to stable conformation. Shine-dalgarno box positions the aug initiation codon for pairing with the trna in bacteria. Pairing of the shine-dalgarno box with the 16s rrna results in a match of the initiation aug codon with the initiator trna (trnai) Positions aug initiation codon in proximity to the ribosome. Have base pairing within the shine-dalgarno sequence and a portion of the 3" end of the 16s rrna sequence (from ribosomal subunit) Base pairing of this sequence with the component of the ribosome (16s rrna) structures that sequence relative to the aug. Ribosome is just like the tata box. Active site of ribosome is on top of aug.
