BIOL 201 Lecture Notes - Lecture 17: Pericentriolar Material, Tubulin, Microtubule-Associated Protein 2
Document Summary
Consists of two centrioles (mother and daughter centriole) Made of microtubules, pericentriolar material, g -turcs: g-turcs ring like structure, templates for tubule nucleation, g-turcs and arp2/3 template by base-plate nucleation; other nucleation mechanism arrange monomers such as spire (binding to the side of actin filament), Only binds to gtp cap growing plus end. Eb1 (end binding) recognizes the relatively blunt end of growing tubules, dcx recognized longitudinal curvature of tubules (when drug is added to straighten the tubule, or introduced mutation, dcx loses binding specificity) Promote tubule growth by enhancing polymerization and reduce catastrophe. Binds and stabilizes a weakly attached tubulin dimer: regulator proteins do not change the critical concentration of polymerization, by changing the concentration, it can catalyst either reverse or forward reaction. Bind and curve the end of tubulin protofilaments into gdp conformation. Hydrolysis of atp is required to remove the terminal dimers (atpase) Can also depolymerize minus end: stathmin proteins.
