BIOL 202 Lecture Notes - Lecture 17: Emor, Dna Replication, Nonsense-Mediated Decay
5 Nov 2015
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Polymerase chain reaction: need to know flanking sequence, heated to denature, cooled to anneal, then re-heated to elongate, ingredients: target dna, dna polymerase, atcg primers. Pcr genotyping of sickle-cell anemia: design primers that flank beta-globin gene, subject dna to mstii digest, it will cut 3 times, but not in the beta-globin gene. Using vntrs and pcr amplification as molecular markers for mapping: can genotype number of repeats by knowing flanking sequence, each pcr gives a different sized product when amplified. Two sources of genetic variation: mutation and recombination of alleles. Somatic vs. germline mutation: germline mutation passes on to the progeny, somatic mutation only affects individual. February 11th, 2015: hypothesis 2: if resistance results from random spontaneous mutation, then resistance can arise at any point before/during/after addition of phage. We should see widely varying (fluctuating) resistance among the samples: spontaneous: background level of mutations, no purposeful or accidental exposure to mutagen o.
