BIOL 300 Lecture 5: BIOL 300 - Lecture 5
5 Mar 2018
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Document Summary
Lecture 5 eukaryotic gene regulatory elements and the transcription factors that bind them. Single-molecule real-time (smrt) sequencing: microscope can read fluorescent of a single molecule, they fix the polymerase at the bottom of the chamber. Dna: sequence is determined in real time, no needs for controlling cycle, can detect the resistance that is provided by each base. The difference of each resistance is an indication of which nucleotide comes in. Transcription factor structure: composed of different domains, dna-binding domain, activation domain, flexible protein domain (they link the dna-binding domains and the activation domain, these domains are modular and can be swapped! Classes of dna-binding domain: helix-turn-helix, two alpha helix linked by a turn, one of the helix is responsible for recognizing dna. It positions itself into the major groove of the dna. 2 zinc finger can bind dna as a homodimer or a heterodimer. It needs to dimerize in order to bind to dna.
