BIOC 212 Lecture Notes - Lecture 9: Vesicle Fusion, Secretion, Signal Transduction
Document Summary
Clathrin-coated vesicles (ccv) : golgi & pm endosomes. Initiation: events on membrane start process of forming vesicle. Adaptors collect tm cargo or cargo receptors. Coat : protein framework formed on top of adaptors shape vesicle bud: fission: separation of vesicle from membrane, uncoating: coat removed allow targeting & fusion. Monomeric gtpase = switches : gtp-bound = binding site for effectors. Gef : release of gdp + binding of gtp. Proteins in er go to golgi and pm by default, even with no exit signal, but many proteins are exported more efficiently/ others return to er. Tm proteins : exit signals on cytosolic side recognized by adaptor. Asp-x-glu (dxe) within a sequence proteins with exit signals ff ) Lumenal proteins : recognized by various cargo receptors (which are tm. Er resident proteins go to golgi by bulk flow, but have signals that return them to er. Lumenal proteins: recognized by tm kdel receptor (kkxx motif at cytosolic.