BIOLOGY 2B03 Lecture Notes - Lecture 9: Cyclin-Dependent Kinase 2, Cyclin-Dependent Kinase 1, Cdc25
11 Dec 2015
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Lecture 2: identifying cell cycle regulators in yeast. Mutated gene codes for a temperature-sensitive protein: folds at permissive temperature but misfolds at restrictive temperatures, change the temperature to turn protein function on and off. Plus = wildtype, minus = loss in function, d = dominant, gain in function. Absence of cdc2: cell fails to divide. Cdc2 protein function increased: cell divides too early and too frequently. Cdc2 protein is a key regulator for entry into mitosis, acting to promote cell division: 32 kda protein with kinase activity, forms a heterodimer with another regulator, cdc13. Cdc13 gene is also a cell cycle regulator: recessive cdc13- = elongated phenotype, dominant cdc13d = wee phenotype, oscillating concentration during the cell cycle, homologous to xenopus cyclin b. Cdc2-cdc13 is a heterodimer = mpf/mitotic cyclin-cdk in fission yeast. Only one type of cdk (cdc2) and one cyclin (cdc13) that functions as the m,s and g-phase cyclin-cdks. Cdc25-: elongated cell, cdc25d: wee cell phenotype.
