BIOL 205 Lecture Notes - Lecture 13: Eif4A, N-Formylmethionine, Bacteriophage
21 Nov 2017
School
Department
Course
Professor
Document Summary
Start slide 26: single nucleotide insertion and deletion can change reading frame, slide 28, wild-type t4 phage can grown on two e. coli strains (b and k). If you take that same phage and then delete a letter, few words are wrong but the reading frame is restored for later words. If the first mutation was a deletion, the suppressor was an insertion or vice versa: the further away the insertion and deletion, the more words are wrong. It is known that something is intermediate between mrna and how the protein is made: which is trna: used a self-free extract, if you take e. coli, and you blend them up and you treat it with. Dna to get (cid:396)id of all the dna, (cid:455)ou"(cid:396)e left (cid:449)ith (cid:449)hate(cid:448)e(cid:396) (cid:449)as i(cid:374) the (cid:272)(cid:455)toplas(cid:373) of the e. coli such as ribosomes, the protein machinery, rna, amino acids and trnas carrying those amino acids.
