BLG 311 Lecture Notes - Lecture 9: Numerical Aperture, Photobleaching, Point Spread Function
8 Feb 2018
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Chapter 9 visualizing cells (579-598; 604-611: a typical animal cell is 10-100 mm in diameter, human eye can only resolve >100 mm particles. Thus the need for instruments that magnify and resolve: microscopes. This is the limit of resolution: magnification: apparent (cid:862)visual(cid:863) increase in the size of an object. There is no limit to magnification: resolution: (cid:862)visual(cid:863) separation of the individual components of an object, which previously appeared as one; quality of the image, resolution limit: is reached when additional magnification does not separate further detail. The result is simply an enlarged blurry image: detection: able to visualize light signals from objects much smaller than 0. 2 mm, including a single molecule. Making use of the wave-properties of light: interference: phase-shifting, diffraction refraction, color, specific organic dyes bind to specific biological molecules, dye indicates presence and amount of biological molecule, before dye staining, tissue samples are processed. Processing of tissue samples is required for staining.
