BIOL 2000 Lecture Notes - Lambda Phage, Lytic Cycle, Bacteriophage
Document Summary
Any bacterial chromosome part is equally likely to get transferred into a new cell. Occasionally phage packages a piece of the bacterial chromosome: any piece of bacteria chromosome is equally likely to get packaged. The ones that packaged bacterial chromosomal dna will infect but not produce new phages. Piece of linear dna into recipient cell. Linear dna can cross over: recombination. A phage particle erroneously packages a host chromosomal dna fragment: size limitation of nda packaged into phage particles. Phage will package a size of chromosomal fragment into the genome fragment from phage: co-transduction of bacterial genes indicates close linkage. If two genes are transferred together means genes are close together. Ability of recipient cell to get 2 genes from donor cell: mapping is similar to transformation analysis. 3 genes are known to be relatively close i a bacterial genome: leu+arg+lac+ What is their relative order o the chromosome: approach: Infect bacterial strain 1: leu+ arg+ lac+ with lytic phage.
