BIOL 2000 Lecture Notes - Stem-Loop, Helicase, Rna Polymerase Iii
Document Summary
Chapter 8-2: for most genes, there will be a single transcription start point and a single end point. 5" start point will always be the same. 3" end point will always be the same. Initiation: start making the rna chain (slow step, governs rate of transcription. Positioned to select where the 5" will be. Elongation: continuous synthesis of the rna chain. Additional sigma protein subunit: core enzyme is elongation and termination steps, holoenzyme is initiation step. Approach: compare many genes and start sites. Where at the same positons (-10) has all the same nucleotides which is t in e. coli. Coding strand of dna is the same as rna but with u for t. E. coli promoters: set of dna sequences that are required to initiate transcription, recognized by rna polymerase and other protein factors, numbering of the nucleotides. Start site = +1 and gets more positive as the polymerase moves. Summary of e. coli: promoter contains two minimal elements.
