NEUR 3625 Lecture Notes - Lecture 1: Peripheral Membrane Protein, Upper Set, Sensory Cortex
Document Summary
Specific components delivered specifically to axonal and dendritic plasma membrane domains. Protein synthesis: correct transmembrane orientation in rer. Integral-membrane proteins: channels, nt receptors, embedded in lipid layer, gpi (glycosylphosphatidylinisitol) - linked proteins, type 1: single transmembrane domain with n-terminus at extracellular surface, type 2: n-terminus at cytoplasmic surface. Integral membrane and secretory proteins synthesized in rer: co-translational insertion in membrane, peripheral membrane proteins, localized on cytoplasmic surface of membrane, do not transverse membrane. The secretory pathway: transport and sorting of proteins in the secretory pathway occur as they pass through the golgi complex before reaching the plasma membrane. Translocation of proteins across rough endoplasmic reticulum (rer) 2 co-translocational modifications in luminal rer: removal of n-terminal hydrophobic signal sequence used for insertion into rer by signal pepsidase, glycosylation: mannose-rich oligosaccharides linked to asparagines through n-acetylglucosamine. For membrane insertions: stop-transfer signal arrests translocation through rer membrane bilayer, sequential display of insertion and stop-transfer signals determines final topology of proteins.
