BIOL 331 Lecture Notes - Lecture 12: Peripheral Membrane Protein, Ankyrin, Endomembrane System

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Eukaryotic proteins are synthesized in two different locations. Group 1: made in free ribosomes in the cytosol. Group 2: made in ribosomes associated with rer. Cytosolic proteins (eg. glycolytic enzymes, proteins of cytoskeleton) Peripheral membrane proteins (eg. spectrins and ankyrins) Proteins that are post-transcriptionally imported into appropriate organelle across membrane, such as: Secreted proteins (cell will send outside, through exocytosis) Soluble proteins of the endomembrane system (er, golgi, lysosomes (vacuoles)) The process of their synthesis is called co-translation translocation (meaning that as proteins are being made, they are being sorted and shipped to various places) Contain built-in (cid:1688)address codes(cid:1689) that allow them to get to final destination. Signal sequence located at or near n-terminus. Special sequence of 6-15 hydrophobic amino acids (first to emerge from ribosome) Directs ribosome with emerging polypeptide to surface of rer. A general scheme to focus our study of the synthesis of group 2 proteins. If you have a signal sequence, you go to rer.

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