CMMB 545 Lecture Notes - Lecture 15: Attenuation, Bioaugmentation, Groundwater Remediation
29 Mar 2017
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Document Summary
Demonstrate mass loss of contaminants: measuring hydrocarbon loss in soils and ground water over time: limitation: loss can be due to non-biological process (dilution, voilatization) Demonstrate site has potential for biodegradation: incubation of environmental samples to determine biodegradation in laboratory: limitation: does not mean activity occurs in situ. Demonstrate potential occurring in situ: measure changes in multiple parameter within the contaminated zone relative to a related uncontaminated area with a tool box : with groundwater monitoring wells placed at different depths are different distances. Toolbox: all techniques can limitation thus needs to use multiple to prove in situ biodegradation. Biogeochemical indicators: monitor chemicals associated with terminal electron accepting processed like electron acceptors (o2, no3. Etc: limitations: no info about current activity status or about hydrocarbon. Etc. ) or respiration products (co2, biodegradation, also ground water can move compounds. Primers for 16s rrna genes or primers for mcra or dsrab gene. Quantitative pcr: allows for the quantification of gene copy number.
