CMMB 411 Lecture Notes - Lecture 11: Dna Replication, Dnab Helicase, Helicase
9 Oct 2018
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5, 9 base pair dnaa binding site, dnaa box. Dnaa binding protein"s affinity (most to least): r4 r1 > r2 > r5 r3. Initiator is the dnaa protein, which will recognize specific proteins and bing them there. Dnaa box is the target site recognized by dnaa protein. A/t means that at this position, you can either put an a or t nucleotide, both are considered good and will be recognized by dnaa protein. If you have cg, this will be a weaker binding site for dnaa protein. Some binding sites don"t match with this sequence, so these sites have different binding sites. Sites 4 and 1 is where dnaa binding protein has affinity to bind first. They define the boundary for where the protein should bind. Ultimate goal is that once dnaa protein binds, they try to build a helical filament of protein. This filament will do something to initiate the melting process. 3,13 base pairs long at rich sequences.
