CMMB 411 Lecture Notes - Lecture 18: Spinal Cord, Gtpase, Prokaryotic Small Ribosomal Subunit

• School: University of Calgary
• Department: Cellular, Molecular and Microbial Biology
• Course: CMMB 411
• Professor: Sui-Lam Wong

Document Summary

Mrna wraps around the neck of the 30s subunit. Entry channel: 12 nucleotides (on the shoulder side) This channel binds and covers 12 nucleotides. Decoding centre: 9 nucleotides (at the centre) Exit channel: 12 nucleotides (on the platform side) 12 nucleotides of rna exit from platform at once. 33 nucleotide long region of mrna is protected by ribosome from nuclease digestion. The 3" end of the 16s rrna is localized at the. Shine-dalgarno region in the picture on the right. L10/l12 stalk will grab trna and push it into the a site. At the base of the l10/l12 stalk, there is a factor binding centre which activates gtpase. The decoding centre (epa sites) is located at the centre of the ribosome. Proteins released all attach to the surface of the mrna. 5" end of rna is localized in this region. Form long extensions which are arginine and lysine rich, positive charge is to neutralize negative charge on rrna.