CMMB 461 Lecture Notes - Lecture 5: National Center For Biotechnology Information, Intron, Gc-Content

• School: University of Calgary
• Department: Cellular, Molecular and Microbial Biology
• Course: CMMB 461
• Professor: Gordon Chua

Document Summary

When you have the plasmids from the shotgun library, you can sequence from both ends. Only the genomic fragment is unique in a vector. Universal primers: only need 2 types of primers because all vectors are the same, so those 2 primers can be used to help sequence all the vectors. Sequences you get from each primer should be close to each other (meaning, they should be very close to each other on the same chromosome) because they are coming from the same fragment. L = 500 x 2 (x2 because these are paired reads) G = 4 mb (4 000 000) N = (1)(4 000 000)/(500 x 2) You"re getting 1x coverage on a 4 mb sequence. Assuming a random library, the sequence coverage of a genome roughly follows a poisson distribution. Y is the number of events in a given interval. Is the mean number of events in a given interval.