CMMB 461 Lecture Notes - Lecture 12: Pax6, Null Allele, Muller'S Morphs
13 Oct 2018
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Delete yfg1, and see the phenotype and based on the phenotype you can infer the function of the gene. By killing or removing the function of yfg, the gene is losing its function, therefore the experiment is called loss-of-function experiment. Over-expressing yfg can make it a mutation. Much more activity of the gene, resulting in lots of protein production. Expect the phenotype of lof and gof experiment to be opposite. Lof mutant: eyeless, eye absent, eye gone. If you knock out any of the 3 genes, and they all have the same function, it can tell you that perhaps the genes function in the same pathway or even in the same complex. The human version of the eyeless gene in flies is pax6. A mutation in pax6 would result in no development of a cornea. In flies, phenotype would be: eyes develop everywhere on the body. Consistent with the gene being required for eye development.
