CMMB 461 Lecture Notes - Lecture 14: Reverse Genetics, Selectable Marker, Design Patterns
18 Nov 2018
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Document Summary
Genome sequences allows for targeted gene perturbation (inactivation/overexpression) and creation of large mutant collections. Systematically alter the function of every gene in the genome (gene deletions/disruptions/overexpression) Analyze these mutants for an abnormal phenotype to determine gene function. Bypasses the need to identify the mutated gene by forward genetic approaches since you know which gene is mutated in every mutant in the collection. Overexpression: gain of function (gof) alleles for every single gene in the genome. Analyze mutants for abnormal phenotype (looking for the phenotype in reverse genetics) Advantage: don"t have to go through transposon tagging and other processes, because you know which gene mutated causes which phenotype. Eukaryote model system, with advanced genetics, molecular biology, biochemistry, and functional genomic methodologies allowing for faster progress in characterizing genes. Many of the cellular processes are conserved in complex multicellular eukaryotes. Genome is first eukaryote to be sequenced (~6000 genes sequenced in 1996) Industrial and biotechnological applications of pathogenic yeast.
