CMMB 511 Lecture Notes - Lecture 2: Gc-Content, Nuclear Dna, Reaction Rate Constant
28 Dec 2019
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Genomic nuclear dna located in nucleus of cells. Genomic complexity is the dna content/haploid genome. Gram positive bacteria have a genomic complexity of 10^6. By doing reassociation kinetics, in which you melt and reassociate ~1000 bp long fragments of dna. Native dna > denatured ssdna > renatured state (fragments become dsdna because they hybridize with complimentary ssdna fragment) Tm: used as reference temperature during reassociation kinetics. High tm = high gc content because at melts faster than gc. Rate of renaturation (hybridization) depends on how repetitive a sequence is. Highly repetitive sequences hybridize faster, therefore have a fast rate of renaturation because they occur multiple times in the genome, therefore there is a higher likelihood of two complimentary strands finding each other. Rate of renaturation data allows us to develop a cot curve. Complexity of dna controls the rate of reassociation of denatured dna. Cot = dna concentration (m) x time (s)
